The collection of protocols that have not yet been curated by IBISBA WP6
Programme: IBISBA
SEEK ID: https://ibisbahub.eu/projects/2
Public web page: http://www.ibisba.eu
Organisms: Pseudomonas putida, Saccharomyces cerevisiae, Escherichia coli, Komagataella (aka pichia) pastoris
IBISBA PALs: No PALs for this Project
Project created: 30th May 2018
Related items
- People (114)
- Programmes (1)
- Institutions (16)
- Data files (0+1)
- Models (0+9)
- SOPs (23+66)
- Publications (3)
- Presentations (0+1)
- Events (1)
- Documents (3)
- Sample types (0+1)
- Samples (0+16)
- Workflows (6+3)
Projects: Uncurated Protocols Library, TRANSCRIPTPROM - Integrative and comparative analyses of response of Pichia pastoris, FLOX: FLavin-containing OXidoreductases, PlanOvac, Muconic Acid Production, HIGHFLUX, Promoters 4 Pichia, An inventory of the Aspergillus niger secretome by combining in silico predictions with shotgun proteomics data, Template library, Evocolour - The evolution and genetics of Flavobacteria forming structurally coloured colonies for sustainable biomaterials, Extreme - Genomic approach for extremozymes screening of thermophilic/anaerobic Caldicoprobacter algerensis strain, HMOzyme, PILOT-MP - Pilot scale process development for microbial proteins, UMB - Upscaling and optimisation of microalgae production for bivalves hatchery, Public announcements and documents, IBISBA Library, Biocare, MEOX, Coculture, YarroFuels, PlasticPutida, Lipro-alga, Curated Protocols Library, IBISBA 1.0 deliverables, PREP-IBISBA deliverables, Cyanofcs, Example testing project
Institutions: Administration, UNIMAN - The University of Manchester
Projects: Uncurated Protocols Library, Yeast Glycerol, Promoters 4 Pichia, IBISBA 1.0 deliverables, PREP-IBISBA deliverables
Institutions: UAB - Universitat Autònoma de Barcelona
https://orcid.org/0000-0001-5626-429XExpertise: Metabolic engineering, metabolic modelling, systems biology, MFA, Mathematical Modelling, Fluxomics, Microbiology, Closed ecologycal systems, Pichia pastoris, photosynthetic bacteria, Kinetic modelling
Tools: Fermentation, Metabolomics, Model organisms, Microbiology, Mathematical Modelling, Matlab, ODE, Computational and theoretical biology, metabolic flux analysis, COBRA, Copasi, RAVEN, metabolic modelling
Projects: Uncurated Protocols Library
Institutions: CSIC - Agencia Estatal Consejo Superior de Investigaciones Cientificas
Projects: Uncurated Protocols Library, MEOX
Institutions: UAB - Universitat Autònoma de Barcelona
https://orcid.org/0000-0002-2924-8902Expertise: Biotechnology, Downstream Processing, Biocatalysis, Multienzymatic Cascade reactions, Cofactor regeneration, enzymatic reactors design, Biocatalytic processes, Biocatalysts Immobilization
Tools: Biocatalysts Immobilization, Enzymatic reaction courses, Enzymatic Activity Assays, Enzymatic Stability Assays
Associated Professor Department of Chemical, Biological and Environmental Engineering Engineering School Universitat Autònoma de Barcelona (UAB)
Projects: Uncurated Protocols Library, FLOX: FLavin-containing OXidoreductases, PlanOvac, HIGHFLUX, SporeDel, Yeast Glycerol, Galambr, SULFOCEL, An inventory of the Aspergillus niger secretome by combining in silico predictions with shotgun proteomics data, Template library, Muconic Acid Production in Yeast, IBISBA 1.0 deliverables, PREP-IBISBA deliverables, Biocare, test, DBTL-P, Upscale of the biocatalytic transformation of Free Fatty Acids into high-value products (BIOFFA), B-PAC, BIOFFA, GLYCOMICS, KorallionBio, MAROX, PROTEIFROM, SUPI
Institutions: UNIMAN - The University of Manchester, UAB - Universitat Autònoma de Barcelona, CNR - Consiglio Nazionale delle Ricerche, INSAT - Institut National des Science Appliquees de Toulouse, VTT - Teknologian tutkimuskeskus VTT Oy, VITO - Vlaamse Instelling voor Technologisch Onderzoek N.V.
IBISBA is designed to accelerate end-to-end bioprocess development, linking best-in-class R&D facilities to provide seamless multi-technology services.
Focusing on the different R&D phases in bioprocess development, IBISBA offers services related to computer-assisted design of biocomponents, construction of enzymes and microbial strains, the fermentation, downstream processing and project knowledge asset management.
Projects: Uncurated Protocols Library, Template library, Muconic Acid Production in Yeast, Public announcements and documents, IBISBA Library, Curated Protocols Library, Test, DBTL-P, test, Sandbox, kHub_Test_2024
Web page: http://www.ibisba.eu
This is a Protocol for transforming plasmid DNA into P. putida cells by electroporation. This is a fast and easy-to-use method of introducing plasmid DNA in P. putida cells with high transformation efficiency. This Protocol is adapted from [1] and encompasses the steps for the preparation of competent cells and transformation of plasmid DNA by electroporation.
All Inputs of the Protocol, listed in the section 4.1., can be acquired either from other IBISBA Protocols or purchased from commercial ...
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: 2 hidden items
Studies: Transformation of production host and 1 hidden item
Assays: 2 hidden items
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Abstract
Authors: Melchior du Lac, Thomas Duigou, Joan Hérisson, Pablo Carbonell, Neil Swainston, Valentin Zulkower, Forum Shah, Léon Faure, Mostafa Mahdy, Paul Soudier, Jean-Loup Faulon
Date Published: 15th Jun 2020
Publication Type: Journal
DOI: 10.1101/2020.06.14.145730
Citation: biorxiv;2020.06.14.145730v1,[Preprint]
Abstract (Expand)
Authors: T. Duigou, M. du Lac, P. Carbonell, J. L. Faulon
Date Published: No date defined
Publication Type: Not specified
PubMed ID: 30321422
Citation: Nucleic Acids Res. 2019 Jan 8;47(D1):D1229-D1235. doi: 10.1093/nar/gky940.
Abstract (Expand)
Authors: B. Delepine, T. Duigou, P. Carbonell, J. L. Faulon
Date Published: No date defined
Publication Type: Not specified
PubMed ID: 29233745
Citation: Metab Eng. 2018 Jan;45:158-170. doi: 10.1016/j.ymben.2017.12.002. Epub 2017 Dec 9.
This meeting brought together ministerial level representatives from 7 European member states to appraise and discuss progress towards launching IBISBA.
Country: France
City: Not specified
This reprot describes achievements so far regarding the construction of IBISBA, a European distributed initiative that aims to provide a world-class infrastructure, enabling cutting-edge research and supporting the development of biotechnology towards a circular economy.
The report was prepared as a support docuement for the 1st Steering Committee meeting that took place online on the 17th June 2021. The document reports progress and provides some indications of next steps in the conception and ...
Creators: Michael O'Donohue, Mauro Di Fenza
Submitter: Michael O'Donohue
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
This document constitutes deliverable 4.4 of the IBISBA 1.0 project. IBISBA 1.0 receives funding from the EU H2020 research and innovation programme under grant agreement No 730976.
Creators: Michael O'Donohue, Julieta Cabello; Laurie Rey; Olivier Rolland
Submitter: Michael O'Donohue
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
A vision paper describing the founding principles behind IBISBA
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Design plasmids encoding predicted pathways by using the BASIC assembly method.
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Given one or a collection of heterolgous pathways in SBML files, generate the SBOL for the constructs and the assembly protocols to build them
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Given a collection of SBML files with the heterologous pathways, calculate the FBA and the Gibbs free energy of the pathways.
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Perform retrosynthesis using RetroPath2.0 and convert the heterologous pathways to SBML files
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Investigations: No Investigations
Studies: No Studies
Assays: No Assays