Transformation of production host

IBISBA offers access to best-in-class facilities and know-how. Clients from academia, SMEs and big industry can find tailored solutions for their R&D needs.

Using a H2020 funding framework, IBISBA currently offers limited opportunities to researchers to obtain subsidised access to IBISBA specialised research structures and facilities, including contribution to user's travel and subsistence costs. Open Calls are launched regularly, inviting researchers from all sectors (academia and industry) ...

Construction of a P. putida KT2440 producing cis,cis-muconate from glucose, benzoate, or p-coumarate

This is a Protocol for transforming plasmid DNA into P. putida cells by electroporation. This is a fast and easy-to-use method of introducing plasmid DNA in P. putida cells with high transformation efficiency. This Protocol is adapted from [1] and encompasses the steps for the preparation of competent cells and transformation of plasmid DNA by electroporation.

All Inputs of the Protocol, listed in the section 4.1., can be acquired either from other IBISBA Protocols or purchased from commercial ...