5L Bioreactor sample
Sample taken from the 5L bioreactors, cell dry weight and HPLC data
Attributes
| Name | Type | Description | PID  | Unit |
|---|---|---|---|---|
| Sample ID* | String | Sample identifier | - | - |
| Sample volume* | Real number | Volume of the sample | - | mL |
| Date time* | Date time | Date and time at which sample was collected | - | - |
| CDW Eppendorf | Real number | Cell dry weight by the eppendorf method | - | g/L |
| CDW Filter | Real number | Cell dry weight by the filter method | - | g/L |
| OD 600nm, 10mm | Real number | Optical density of sample at 600nm, and light path of 10 mm | - | a.u. |
| Glucose YSI | Real number | Glucose concentration by enzymatic analyzer YSI | - | g/L |
| Glucose HPLC | Real number | Glucose concentration by HPLC | - | g/L |
| Aetate HPLC | Real number | Acetate concentration by HPLC | - | g/L |
| Citrate HPLC | Real number | Citrate concentration by HPLC | - | g/L |
| Nanobody densitometry | Real number | Nanobody concentration (mg/L) by densitometry in electrophoresis gel | - | - |
| Nanobody nanodrop | Real number | Nanobody concentration (mg/L) by Nanodrop (280 nm) | - | - |
| Glucose feed* | Real number | Concentration of the glucose feed | - | g/L |
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Created: 29th Apr 2025 at 13:35
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Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH), Production of the monoclonal antibody Trastuzumab in Komagataella phaffii (Pichia pastoris) for breast cancer treatment
Institutions: INSAT - Institut National des Science Appliquees de Toulouse, INRAE
https://orcid.org/0000-0001-8290-1635
Bioprocess engineer with extensive expertise in bioreactor process design, fermentation technologies, and protein production. Skilled in scaling up bioprocesses, experimental design, and process optimization.
Bioindustry 4.0 is a cutting-edge initiative perfecting advanced technologies to empower European bioindustry sector. Our overarching aim is to make the development of bioprocesses faster and cheaper, and biomanufacturing more reliable.
Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH), Production of the monoclonal antibody Trastuzumab in Komagataella phaffii (Pichia pastoris) for breast cancer treatment
Web page: https://www.bioindustry4.eu
Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
