Bioindustry 4.0

Industrial-scale penicillin simulation

Industrial-scale penicillin simulation (IndPenSim) is a first principles mathematical model of a 100,000 Litre fermentation of an industrial strain of Penicillium chrysogenum validated using historical data collected from an industrial-scale penicillin fermentation process. Adapting a previously published structured model to describe penicillin fermentation and extending the model to include the main environmental effects of dissolved oxygen, ...

Hybrid Physics-Informed Neural Networks for Efficient Computational Modeling: A Workflow Framework Applied to Schwanniomyces occidentalis

Bioindustry4.0 Work Package 7: Tools for high-quality datasets, requisite for data-driven methods and services

Real-time online monitoring of bioprocesses

Industrial biotechnology (IB) has the potential to create new markets while protecting the environment. With this in mind, the EU-funded BIOINDUSTRY 4.0 project will pave the way for IB to become a major manufacturing technology. Supporting the digitalisation of IB, the project will create new services delivered by European research ...

Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...

We studied the production of scorpion antivenom nanobodies in high cell density microbioreactors. The BiolectorXT (Beckman) was used to screen different production strategies within the framework of a machine-learning model optimization.

In the biopharmaceutical industry, nanobodies show promising properties for its application in serotherapy for serums targeting the most toxic components of scorpion venom. Nevertheless, the limited number of scientific publications on the production of nanobodies for antivenom productions poses a challenge. The reported yield is low (< 4 mg/L), biomass production is not reported, and the production in high cell density cultures have not been studied. Production conditions (temperature of ...

The screening of E. coli strains WK6, CH10-12 (2017), CH10-12 (2023), NbF12-10 (2017), and NbF12-10 (2023) was made in a BioLectorXT (Beckman) setting

Culture of E. coli strains WK6, CH10-12 (2017), CH10-12 (2023), NbF12-10 (2017), and NbF12-10 (2017) at 28°C in a fed-batch microbioreactor setting.

5L bioreactor culture of strain E. coli WK6 in a batch/fed-batch strategy at 37°C. Characterization of biomass growth and oxygen transfer

5L bioreactor culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C (BR02). Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 38 h

5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h

32 fed-batch cultures at 28°C

Excel file containing the data anlysis of the BR03 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR04 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR05 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR06 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

The IBISBA Knowledge Hub (IBISBAKHub) is a data management platform designed to help researchers enhance the management of their research data. It offers an improved way to share, register, and organize the data and assets generated from research projects. The IBISBAKHub’s primary objective is to promote the adoption and implementation of FAIR Data principles.

IBISBA DIALS - Funded by the European Union (Grant agreement N°101131085). Views and opinions expressed are however those of the author(s) ...

The IBISBA Knowledge Hub (IBISBAKHub) is a data management platform designed to help researchers enhance the management of their research data. It offers an improved way to share, register, and organize the data and assets generated from research projects. The IBISBAKHub’s primary objective is to promote the adoption and implementation of FAIR Data principles.

IBISBA DIALS - Funded by the European Union (Grant agreement N°101131085). Views and opinions expressed are however those of the author(s) ...

Global results obtained from the 5 L bioreactor cultures of E. coli

The stakeholder workshop on IoT: Data integration from the lab took place on October 23rd, 2025 from 10:00-12:00 (CET). This two-hour workshop brought together experts and stakeholders to explore how laboratory data can be better integrated, managed, and applied using Internet of Things (IoT) technologies. The purpose of this event was to foster dialogue on the opportunities but also the challenges of IoT data acquisition within laboratory environments, exploring how connected equipment and sensors ...

5L bioreactor culture of_ E. coli _NbF12-10 at 29°C in nanobody production phase adding 1 mM IPTG to the bioreactor. Caracterization of nanobody production in glucose-limited conditions, using a constant glucose feed strategy of 4.5 g/h

5L bioreactor culture of E. coli WK6 at 29°C in biomass production phase under induction conditions adding 1 mM IPTG to the bioreactor. Caracterization of biomass production in glucose-limited conditions, using a constant glucose feed strategy of 4.5 g/h

5L bioreactor culture of E. coli NbF12-10 at 37°C in fed-batch phase. Caracterization of biomass production phase in glucose-limited conditions, using an exponential-like glucose feed strategy for a µ=0.38 h-1

5L bioreactor culture of_ E. coli_ WK6 at 37°C in fed-batch phase. Caracterization of biomass production phase in glucose-limited conditions, using an exponential-like glucose feed strategy for a µ=0.38 h-1

Procedure followed for the data treatment and analysis of 5L bioreactor cultures

Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR04 Channel 4: gas out - BR05 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR02 Channel 4: gas out - BR03 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s

5L bioreactor of E. coli NbF12-10 in a batch/fed-batch strategy at 37°C, induction promoted for 37h at 29°C MFCS BioPAT Sartorius

5L bioreactor of E. coli WK6 in a batch/fed-batch strategy at 37°C, induction promoted for 28h at 29°C MFCS BioPAT Sartorius

5L bioreactor of E. coli CH10-12 in a batch/fed-batch strategy at 37°C, induction promoted for 35h at 32°C MFCS BioPAT Sartorius