Culture BR02 - induction phase
Nanobody production phase, IPTG 1 mM added to the bioreactor
SEEK ID: https://ibisbahub.eu/events/8
Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant ...
16th May 2018 at 22:25 (17th May 2018 at 00:25 (Europe/Paris))
17th May 2018 at 10:42 (17th May 2018 at 12:42 (Europe/Paris))
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Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
Programme: This Project is not associated with a Programme
Public web page: Not specified
Start date: 6th Feb 2017
Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR02 Channel 4: gas out - BR03 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 28°C for 12h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 28°C for 12h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
