5L bioreactor culture of strain E. coli WK6 at 37°C. Characterization of biomass growth and oxygen transfer in the batch phase
SEEK ID: https://ibisbahub.eu/events/5
Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant ...
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15th Mar 2018 at 14:04 (15th Mar 2018 at 15:04 (Europe/Paris))
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Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
Programme: This Project is not associated with a Programme
Public web page: Not specified
Start date: 6th Feb 2017
Organisms: Escherichia coli
Samples taken in the 5L bioreactor BR01 in the culture of E. coli WK6 in batch and fed-batch strategy
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 4: gas out - BR01 Channel 6: gas in (reference) Sampling time: 60 s Flushing time: 45 s Analysis time: 15 s
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Excel file containing the data anlysis of the BR01 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Bioreactor data acquisition - part 1
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela