BiolectorXT data from the screening of E. coli strains Version 1
32 fed-batch cultures at 28°C
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Created: 20th Oct 2025 at 14:35
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Version 1 (earliest) Created 20th Oct 2025 at 14:35 by Susana Alonso Villela
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Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH), Production of the monoclonal antibody Trastuzumab in Komagataella phaffii (Pichia pastoris) for breast cancer treatment
Institutions: INSAT - Institut National des Science Appliquees de Toulouse, INRAE
https://orcid.org/0000-0001-8290-1635
Bioprocess engineer with extensive expertise in bioreactor process design, fermentation technologies, and protein production. Skilled in scaling up bioprocesses, experimental design, and process optimization.
Bioindustry 4.0 is a cutting-edge initiative perfecting advanced technologies to empower European bioindustry sector. Our overarching aim is to make the development of bioprocesses faster and cheaper, and biomanufacturing more reliable.
Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH), Production of the monoclonal antibody Trastuzumab in Komagataella phaffii (Pichia pastoris) for breast cancer treatment
Web page: https://www.bioindustry4.eu
Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
We studied the production of scorpion antivenom nanobodies in high cell density microbioreactors. The BiolectorXT (Beckman) was used to screen different production strategies within the framework of a machine-learning model optimization.
The screening of E. coli strains WK6, CH10-12 (2017), CH10-12 (2023), NbF12-10 (2017), and NbF12-10 (2023) was made in a BioLectorXT (Beckman) setting
Submitter: Susana Alonso Villela
Investigation: Scale-down effects of the nanobody producing E....
Assays: Fed-batch culture of E. coli strains in microbioreactors
Culture of E. coli strains WK6, CH10-12 (2017), CH10-12 (2023), NbF12-10 (2017), and NbF12-10 (2017) at 28°C in a fed-batch microbioreactor setting.
Submitter: Susana Alonso Villela
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Scale-down effects of the nanobody producing E....
