Conjugation procedure of pK18mobSacB plasmid from E. coli S17pir strain to P. putida KT2440 strain

This protocol describes the procedure of conjugation of pK18mobSacB suicide plasmid containing fragments UP and DOWN that delimit the recombination region from E. coli S17λpir to P. putida cells.

SEEK ID: https://ibisbahub.eu/sops/120?version=2

Filename: Conjugation procedure of pK18mobSacB plasmid from E. coli S17pir strain to P. putida KT2440 strain.docx 

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Laura de Eugenio, Beatriz Galán

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Creative Commons Attribution 4.0 International
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Created: 4th Aug 2021 at 16:29

Last updated: 4th Aug 2021 at 16:30

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Version 2 (latest) Created 4th Aug 2021 at 16:30 by Felipe de Salas

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Version 1 (earliest) Created 4th Aug 2021 at 16:29 by Felipe de Salas

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