Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
Programme: This Project is not associated with a Programme
Public web page: Not specified
Start date: 6th Feb 2017
Organisms: Escherichia coli
Submitter: Susana Alonso Villela
Investigation: Effect of the induction temperature in nanobody...
Assays: Fed-batch culture of WK6 strain, without induction - BR01
Snapshots: No snapshots
5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 32°C for 35 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 38 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 37°C for 6 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 33°C for 6 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C (BR02). Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BioPAT MFCS data for BR02 - part 1, BioPAT MFCS data for BR02 - part 2, Gas analysis data for BR02 and BR03
Snapshots: No snapshots
5L bioreactor culture of strain E. coli WK6 in a batch/fed-batch strategy at 37°C. Characterization of biomass growth and oxygen transfer
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR01 samples, BioPAT MFCS data for BR01 - part 1, BioPAT MFCS data for BR01 - part 2, Data analysis of the BR01, Gas analysis data for BR01
Snapshots: No snapshots
Samples taken in the 5L bioreactor BR01 in the culture of E. coli WK6 in batch and fed-batch strategy
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: 1 hidden item
Studies: 1 hidden item
Assays: 1 hidden item
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Abstract (Expand)
Authors: Susana María Alonso Villela, Hazar Ghezal-Kraïem, Balkiss Bouhaouala-Zahar, Carine Bideaux, César Arturo Aceves Lara, Luc Fillaudeau
Date Published: 1st Feb 2021
Publication Type: Journal
DOI: 10.1007/s00253-021-11093-5
Citation: Appl Microbiol Biotechnol 105(3):1017-1030