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2 Publications visible to you, out of a total of 2
An inventory of the Aspergillus niger secretome by combining in silico predictions with shotgun proteomics data

Abstract (Expand)

Background The ecological niche occupied by a fungal species, its pathogenicity and its usefulness as a microbial cell factory to a large degree depends on its secretome. Protein secretion usually …y requires the presence of a N-terminal signal peptide (SP) and by scanning for this feature using available highly accurate SP-prediction tools, the fraction of potentially secreted proteins can be directly predicted. However, prediction of a SP does not guarantee that the protein is actually secreted and current in silico prediction methods suffer from gene-model errors introduced during genome annotation. Results A majority rule based classifier that also evaluates signal peptide predictions from the best homologs of three neighbouring Aspergillus species was developed to create an improved list of potential signal peptide containing proteins encoded by the Aspergillus niger genome. As a complement to these in silico predictions, the secretome associated with growth and upon carbon source depletion was determined using a shotgun proteomics approach. Overall, some 200 proteins with a predicted signal peptide were identified to be secreted proteins. Concordant changes in the secretome state were observed as a response to changes in growth/culture conditions. Additionally, two proteins secreted via a non-classical route operating in A. niger were identified. Conclusions We were able to improve the in silico inventory of A. niger secretory proteins by combining different gene-model predictions from neighbouring Aspergilli and thereby avoiding prediction conflicts associated with inaccurate gene-models. The expected accuracy of signal peptide prediction for proteins that lack homologous sequences in the proteomes of related species is 85%. An experimental validation of the predicted proteome confirmed in silico predictions.

Authors: Machtelt Braaksma, Elena S Martens-Uzunova, Peter J Punt, Peter J Schaap

Date Published: 1st Dec 2010

Publication Type: Journal

Towards a machine learning operations (MLOps) soft sensor for real-time predictions in industrial-scale fed-batch fermentation

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Real-time predictions in fermentation processes are crucial because they enable continuous monitoring and control of bioprocessing. However, the availability of online measurements is limited by the … availability and feasibility of sensing technology. Soft sensors - or software sensors that convert available measurements into measurements of interest (product yield, quality, etc.) - have the potential to improve efficiency and product quality. Machine learning (ML) based soft sensors have gained increased popularity over the years since they can incorporate variables that are measured in real-time, and exploit the intricate patterns embedded in such voluminous datasets. However, ML-based soft sensor requires more than just a classical ML learner with an unseen test set to evaluate the quality prediction of the model. When a ML model is deployed in production, its performance can deteriorate rapidly leading to an unanticipated decline in the quality of the output and predictions. Here a proof concept of Machine Learning Operations (MLOps) to automate the end-to-end soft sensor lifecycle in industrial scale fed-batch fermentation, from development and deployment to maintenance and monitoring is proposed. Using the industrial-scale penicillin fermentation (IndPenSim) dataset that includes 100 fermentation batches, to build a soft sensor based on Long Short Term Memory (LSTM) for penicillin concentration prediction. The batches containing deviations in the processes (91–100) were used to assess concept drift of the LSTM soft sensor. The evaluation of concept drift is evidenced by the soft sensor performance falling below the set threshold based on the Population Stability Index (PSI), which automatically triggers an alert to run the retraining pipeline.

Authors: Brett Metcalfe, Juan Camilo Acosta-Pavas, Carlos Eduardo Robles-Rodriguez, George K. Georgakilas, Theodore Dalamagas, Cesar Arturo Aceves-Lara, Fayza Daboussi, Jasper J Koehorst, David Camilo Corrales

Date Published: 1st Mar 2025

Publication Type: Journal

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