Proteins detected by high-throughput mass spectrometry of secretome enriched fractions of A. niger N402 cultured under a set of controlled conditions

Selected Cell

Cell:

Value:

detected proteins

100

101

102

103

104

105

106

107

108

109

110

111

112

113

114

115

116

117

118

119

120

121

122

123

124

125

126

127

128

129

130

131

132

133

134

135

136

137

138

139

140

141

142

143

144

145

146

147

148

149

150

151

152

153

154

155

156

157

158

159

160

161

162

163

164

165

166

167

168

169

170

171

172

173

174

175

176

177

178

179

180

181

182

183

184

185

186

187

188

189

190

191

192

193

194

195

196

197

198

199

200

# Proteins detected by high-throughput mass spectrometry of secretome enriched fractions of A. niger N402 cultured under a set of controlled conditions

No.	Functional group	Description	Locus tag	SignalP3 prediction	SP according to classifier * (see sup. file 1)	Spectral counts on sorbitol growth conditions (t-24 and t-48)	Spectral counts on GalA growth conditions (t-24 and t-48)	Spectral counts under starvation conditions (all 9 samples)	MEROPS peptidase family	CAZy family	Predicted GPI-anchored protein	Functional classification of ambiguous and likely SP proteins in the SignalP3 defined non-SP group
1	CARBOHYDRATE	endo-14-xylanase xynB - Aspergillus niger	An01g00780	SP	yes	0	0	12		Glycoside Hydrolase Family 11
2	ACTIVE	strong similarity to alphaalpha-trehalase treA - Aspergillus nidulans	An01g01540	SP	likely SP	10	10	0		Glycoside Hydrolase Family 65
3	ENZYMES	strong similarity to 13-beta-glucanosyltransferase gel1 - Aspergillus fumigatus	An01g03090	SP	likely SP	1	0	1		Glycoside Hydrolase Family 81
4		strong similarity to mixed-linked glucanase precursor MLG1 - Cochliobolus carbonum-	An01g04560	SP	likely SP	0	0	4		Glycoside Hydrolase Family 16
5		strong similarity to alpha-L-rhamnosidase A precursor rhaA - Aspergillus aculeatus-	An01g06620	SP	yes	0	7	0		Glycoside Hydrolase Family 78
6		xylosidase xlnD - Aspergillus niger-	An01g09960	SP	yes	5	23	100		Glycoside Hydrolase Family 03
7		strong similarity to enzyme with sugar transferase activity from patent JP11009276-A - Acremonium sp.-	An01g10930	SP	yes	3	4	1		Glycoside Hydrolase Family 31
8		14-beta-D-glucan cellobiohydrolase B precursor cbhB - Aspergillus niger	An01g11660	SP	yes	1	0	1		Glycoside Hydrolase Family 7
9		strong similarity to hypothetical glucan beta-13 exoglucanase exgS - Aspergillus phoenicis	An01g12450	SP	yes	16	12	10		Glycoside Hydrolase Family 55
10		strong similarity to mannosyl-oligosaccharide 12-alpha-mannosidase msdS - Aspergillus saitoi	An01g12550	SP	yes	95	71	13		Glycoside Hydrolase Family 47
11		alpha-galactosidase aglB - Aspergillus niger-	An02g11150	SP	yes	25	7	2		Glycoside Hydrolase Family 27
12		strong similarity to diglycosidase related protein SEQ ID NO:10 from patent WO200018931-A1 - Aspergillus fumigatus-	An03g00500	SP	yes	16	15	15		Glycoside Hydrolase Family 30
13		endo-14-beta-xylanase A precursor xynA - Aspergillus niger [putative sequencing error]	An03g00940	SP	yes	0	0	2		Glycoside Hydrolase Family 10
14		14-beta-D-arabinoxylan arabinofuranohydrolase axhA - Aspergillus niger	An03g00960	SP	likely SP	1	0	6		Glycoside Hydrolase Family 62
15		similarity to endo-beta-14-glucanase - Bacillus polymyxa	An03g01050	SP	yes	1	0	0		Glycoside Hydrolase Family 05
16		similarity to cellulase #2 from patent US2003036176-A1 - Xanthomonas campestris-	An03g04190	SP	yes	3	0	0		expansin-like protein
17		similarity to glucan 13-beta-glucosidase Bgl2 - Saccharomyces cerevisiae	An03g05290	SP	yes	113	54	86		Glycoside Hydrolase Family 17
18		strong similarity to endo-beta-14-glucanase EGIII-like from patent WO9931255-A2 - Emericella desertoru	An03g05530	SP	yes	2	3	0		Glycoside Hydrolase Family 12
19		glucan 14-alpha-glucosidase glaA - Aspergillus niger	An03g06550	SP	yes	175	19	132		Glycoside Hydrolase Family 15
20		strong similarity to beta-glucosidase bglB - Candida wickerhamii-	An04g03170	SP	ambiguous	2	0	0		Glycoside Hydrolase Family 01
21		extracellular alpha-glucosidase aglU - Aspergillus niger-	An04g06920	SP	yes	81	3	18		Glycoside Hydrolase Family 31
22		strong similarity to extracellular alpha-amylase amyA/amyB - Aspergillus niger-	An04g06930	SP	yes	4	1	0		Glycoside Hydrolase Family 13
23		strong similarity to hypothetical protein CC0812 - Caulobacter crescentus-	An04g09360	SP	yes	1	3	0		Carbohydrate Esterase Family 12
24		alpha-galactosidase aglA - Aspergillus niger-	An06g00170	SP	yes	9	0	10		Glycoside Hydrolase Family 27
25		strong similarity to exo-13-beta-glucanase KlEXG1 - Kluyveromyces lactis	An08g01100	SP	yes	2	3	0		Glycoside Hydrolase Family 05
26		strong similarity to xylan 14-beta-xylosidase - Butyrivibrio fibrisolvens	An08g01900	SP	ambiguous	0	0	8		Glycoside Hydrolase Family 43
27		strong similarity to hypothetical protein CC0533 - Caulobacter crescentus-	An08g03060	SP	yes	0	0	2		Glycoside Hydrolase Family 92
28		strong similarity to 13-beta-glucanosyltransferase bgt1 - Aspergillus fumigatus	An08g03580	SP	yes	13	5	0		Glycoside Hydrolase Family 17
29		strong similarity to hypothetical endoglucanase IV - Trichoderma reesei-	An08g05230	SP	yes	12	1	2		Glycoside Hydrolase Family 61
30		similarity to alpha-12-mannosidase aman2 - Bacillus sp.	An08g08370	SP	yes	4	0	3		Glycoside Hydrolase Family 92
31		beta-fructofuranosidase precursor suc1 - Aspergillus niger-	An08g11070	SP	yes	0	6	0		Glycoside Hydrolase Family 32
32		alpha-galactosidase aglC - Aspergillus niger [truncated ORF][putative sequencing error]	An09g00260	SP	yes	2	8	1		Glycoside Hydrolase Family 36
33		strong similarity to beta-N-acetylhexosaminidase from patent WO9839459-A1 - Penicillium chrysogenum-	An09g02240	SP	yes	0	0	2		Glycoside Hydrolase Family 20
34		strong similarity to alpha-xylosidase XylS - Sulfolobus solfataricus-	An09g03300	SP	yes	0	5	7		Glycoside Hydrolase Family 31
35		strong similarity to beta-glucosidase bgln - Candida molischiana-	An11g00200	SP	yes	0	1	0		Glycoside Hydrolase Family 03
36		inulinase inuA - Aspergillus niger-	An11g03200	SP	yes	1	1	0		Glycoside Hydrolase Family 32
37		acid alpha-amylase - Aspergillus niger[putative sequencing error]	An11g03340	SP	yes	47	1	27		Glycoside Hydrolase Family 13
38		beta-mannosidase mndA - Aspergillus niger[putative sequencing error]	An11g06540	SP	likely SP	0	12	2		Glycoside Hydrolase Family 02
39		strong similarity to alpha-amylase - Aspergillus niger-	An12g02460	SP	yes	5	10	0		Glycoside Hydrolase Family 13
40		exo-inulinase inu1 - Aspergillus niger-	An12g08280	SP	ambiguous	12	3	0		Glycoside Hydrolase Family 32
41		weak similarity to heparanase - Homo sapiens-	An14g01620	SP	ambiguous	5	19	0		Glycoside Hydrolase Family 79
42		strong similarity to beta-glucosidase bgln - Candida molischiana-	An14g01770	SP	yes	0	20	0		Glycoside Hydrolase Family 03
43		strong similarity to alpha-galactosidase - Cyamopsis tetragonoloba-	An14g01800	SP	likely SP	7	8	5		Glycoside Hydrolase Family 27
44		strong similarity to endoglucanase IV egl4 - Trichoderma reesei-	An14g02670	SP	yes	2	0	1		Glycoside Hydrolase Family 61
45		endoglucanase A eglA - Aspergillus niger-	An14g02760	SP	yes	0	0	6		Glycoside Hydrolase Family 12
46		alpha-glucuronidase aguA - Aspergillus niger-	An14g05800	SP	yes	0	0	3		Glycoside Hydrolase Family 67
47		strong similarity to beta-12-D-glucosidase B2Tom - Septoria lycopersici	An15g04800	SP	yes	0	3	0		Glycoside Hydrolase Family 03
48		strong similarity to precursor of alpha-amylase A - Aspergillus niger-	An15g07800	SP	yes	13	3	0		Glycoside Hydrolase Family 13
49		weak similarity to endo-14-beta-xylanase CAA93120.1 - Ascochyta pisi	An16g03330	SP	yes	15	14	7		thaumatin-like protein
50		strong similarity to endoglucanase eglB - Aspergillus niger-	An16g06800	SP	yes	31	25	5		Glycoside Hydrolase Family 05
51		beta-glucosidase bgl1 - Aspergillus niger-	An18g03570	SP	yes	99	92	148		Glycoside Hydrolase Family 03
52		strong similarity to 43 kDa secreted glycoprotein precursor gp43 - Paracoccidioides brasiliensis-	An18g04100	SP	likely SP	1	3	2		Glycoside Hydrolase Family 05
total counts per group						835	475	644

53	CARBOHYDRATE	alpha-l-arabinofuranosidase a precursor abfA - Aspergillus niger-	An01g00330	SP	yes	0	5	13		Glycoside Hydrolase Family 51
54	ACTIVE	strong similarity to secreted beta-galactosidase lacA - Aspergillus niger-	An01g10350	SP	yes	2	37	0		Glycoside Hydrolase Family 35
55	ENZYMES,	polygalacturonase pgaI - Aspergillus niger-	An01g11520	SP	yes	0	2	0		Glycoside Hydrolase Family 28
56	PECTIN	beta-galactosidase lacA - Aspergillus niger-	An01g12150	SP	yes	4	45	8		Glycoside Hydrolase Family 35
57	RELATED	polygalacturonase E precursor pgaE - Aspergillus niger-	An01g14670	SP	yes	0	3	0		Glycoside Hydrolase Family 28
58		strong similarity to acetyl-esterase I from patent WO9502689-A - Aspergillus aculeatus-	An02g02540	SP	yes	0	19	1		Carbohydrate Esterase Family 16
59		endopolygalacturonases pgaB - Aspergillus niger-	An02g04900	SP	yes	4	7	2		Glycoside Hydrolase Family 28
60		strong similarity to endo-alpha-15-arabinanase abnA - Aspergillus niger	An02g10550	SP	yes	41	32	12		Glycoside Hydrolase Family 43
61		similarity to polygalacturonase XOPG1 - Lycopersicon esculentum-	An02g12450	SP	yes	0	19	0		Glycoside Hydrolase Family 28
62		similarity to pectin methylesterase pmeA - Aspergillus oryzae-	An02g12505	SP	yes	2	89	0		Carbohydrate Esterase Family 08
63		pectinesterase pmeA - Aspergillus niger-	An03g06310	SP	yes	2	47	0		Carbohydrate Esterase Family 08
64		strong similarity to exo-alpha 14-polygalacturonase PGX1 - Cochliobolus carbonum	An03g06740	SP	yes	0	40	0		Glycoside Hydrolase Family 28
65		strong similarity to pectin methylesterase pme1 - Aspergillus aculeatus-	An04g09690	SP	yes	2	16	0		Carbohydrate Esterase Family 08
66		strong similarity to endo-xylogalacturonan hydrolase xghA - Aspergillus tubingensis-	An04g09700	SP	yes	0	4	0		Glycoside Hydrolase Family 28
67		strong similarity to beta-galactosidase lacA - Aspergillus niger-	An06g00290	SP	yes	0	8	0		Glycoside Hydrolase Family 35
68		strong similarity to hypothetical protein T16K5.230 - Arabidopsis thaliana-	An08g10780	SP	yes	9	9	3		Glycoside Hydrolase Family 43
69		ferulic acid esterase A faeA - Aspergillus niger-	An09g00120	SP	yes	0	0	2		Carbohydrate Esterase Family 01
70		similarity to acetyl xylan esterase precursor axe1 - Trichoderma reesei-	An09g00790	SP	yes	1	0	0		cutinase-like protein
71		strong similarity to alpha-L-arabinofuranosidase A abfA - Aspergillus niger-	An09g00880	SP	ambiguous	0	8	0		Glycoside Hydrolase Family 51
72		endo 15-alpha-arabinanase abnA - Aspergillus niger	An09g01190	SP	yes	1	0	0		Glycoside Hydrolase Family 43
73		rhamnogalacturonan acetyl esterase rgaeA - Aspergillus niger-	An09g02160	SP	yes	1	0	0		SGNH hydrolase protein
74		endo-polygalacturonase D pgaD - Aspergillus niger-	An09g03260	SP	yes	0	3	0		Glycoside Hydrolase Family 28
75		acetyl xylan esterase axeA - Aspergillus niger-	An12g05010	SP	yes	0	0	1		Carbohydrate Esterase Family 01
76		strong similarity to exopolygalacturonase pgaX - Aspergillus tubingensis-	An12g07500	SP	yes	0	18	0		Glycoside Hydrolase Family 28
77		rhamnogalacturonase rhgB - Aspergillus niger-	An14g04200	SP	yes	4	2	0		Glycoside Hydrolase Family 28
78		pectin lyase pelA - Aspergillus niger-	An14g04370	SP	yes	0	8	0		Polysaccharide Lyase Family 01
79		arabinofuranosidase B abfB - Aspergillus niger-	An15g02300	SP	yes	61	99	22		Glycoside Hydrolase Family 54
80		polygalacturonase pgaII - Aspergillus niger-	An15g05370	SP	yes	1	5	0		Glycoside Hydrolase Family 28
81		endo-polygalacturonase A pgaA - Aspergillus niger [putative frameshift][putative frameshift]	An16g06990	SP	yes	0	1	0		Glycoside Hydrolase Family 28
82		strong similarity to exo-polygalacturonase PGX from patent WO9414966-A - Aspergillus tubingensis-	An18g04810	SP	likely SP	0	2	0		Glycoside Hydrolase Family 28
83		strong similarity to arabinogalactan endo-14-beta-galactosidase gal1 - Aspergillus aculeatus	An18g05940	SP	yes	0	4	0		Glycoside Hydrolase Family 53
84		pectin lyase pelD - Aspergillus niger-	An19g00270	SP	yes	0	1	0		Polysaccharide Lyase Family 01
85		arabinofuranosidase E abfE - Aspergillus niger	ACE00420	SP	yes	0	7	0		Glycoside Hydrolase Family 51
total counts per group						135	540	64

86	PROTEASES/	sim. to lysosomal protease CLN2 - Rattus norvegicus-	An01g01750	SP	yes	14	5	24	peptidase family S53
87	PEPTIDASES	s. sim. to serine-type carboxypeptidase I cdpS - Aspergillus saitoi[putative sequencing error]	An02g04690	SP	yes	9	5	3	peptidase family S10
88		aspartic protease pepE - Aspergillus niger-	An02g07210	SP	yes	0	0	2	peptidase family A1
89		s. sim. to lysosomal pepstatin insensitive protease CLN2 - Homo sapiens-	An03g01010	SP	yes	9	10	2	peptidase family S53
90		s. sim. to vacuolar aminopeptidase Y Ape3 - Saccharomyces cerevisiae-	An03g01660	SP	yes	0	8	3	peptidase family M28
91		s. sim. to carboxypeptidase S1 - Penicillium janthinellum-	An03g05200	SP	yes	38	21	49	peptidase family S10
92		s. sim. to precursor of pepsin A3 - Homo sapiens-	An04g01440	SP	yes	0	0	2	peptidase family A1
93		s. sim. to lysosomal pepstatin insensitive protease CLN2 - Homo sapiens-	An06g00190	SP	yes	7	16	68	peptidase family S53
94		endoprotease Endo-Pro - Aspergillus niger-	An08g04490	SP	yes	58	25	53	peptidase family S28
95		s. sim. to hypothetical lysosomal pepstatin insensitive protease CLN2 - Canis lupus-	An08g04640	SP	yes	17	4	24	peptidase family S53
96		s. sim. to aspartic protease pr1 - Phaffia rhodozyma-	An12g03300	SP	yes	0	1	0	peptidase family A1
97		s. sim. to dipeptidyl peptidase II DPPII - Rattus norvegicus-	An12g05960	SP	yes	42	20	29	peptidase family S28
98		s. sim. to aspartic proteinase Yps3 - Saccharomyces cerevisiae-	An13g02130	SP	likely SP	1	1	0	peptidase family A1
99		s. sim. to serine-type carboxypeptidase precursor cpdS - Aspergillus phoenicis-	An14g02150	SP	yes	0	1	4	peptidase family S10
100		s. sim. to protein PRO304 from patent WO200104311-A1 - Homo sapiens-	An14g02470	SP	yes	18	18	67	peptidase family S53
101		aspartic proteinase aspergillopepsin I pepA - Aspergillus niger-	An14g04710	SP	yes	87	33	226	peptidase family A1
102		s. sim. to aspartic proteinase aspergillopepsin I pepA - Aspergillus niger [truncated ORF]-	An15g06280	SP	yes	4	0	0	peptidase family A1
103		s. sim. to extracellular protease precursor Bar1 - Saccharomyces cerevisiae-	An18g01320	SP	yes	9	8	17	peptidase family A1
104		putative aspartic-type endo peptidase, secreted, not in CBS	ATCC 53364	SP	yes	2	3	4	peptidase family A1
total counts per group						315	179	577

105	LIPASES/	sim. to nonhemolytic phospholipase C PC-PLC - Burkholderia pseudomallei-	An01g14940	SP	yes	14	0	42
106	ESTERASES	s. sim. to lysophospholipase phospholipase B - Penicillium notatum-	An02g13220	SP	ambiguous	0	8	9
107		s. sim. to cephalosporin esterase - Rhodosporidium toruloides-	An02g14290	SP	yes	9	6	0
108		sim. to lipase LIP1 - Candida rugosa-	An03g06630	SP	yes	0	3	0
109		s. sim. to lysophospholipase from patent WO0127251 - Aspergillus oryzae-	An04g03590	SP	yes	0	1	0
110		s. sim. to secretory lipase LIP2 - Candida albicans-	An07g00440	SP	yes	3	5	6
111		s. sim. to butyrylcholinesterase BChE - Oryctolagus cuniculus-	An07g04470	SP	yes	0	1	0
112		s. sim. to phospholipase B - Penicillium notatum-	An09g01240	SP	yes	2	14	11
113		sim. to lipase lip1 - Geotrichum candidum-	An09g05120	SP	ambiguous	1	1	0
114		s. sim. to triacylglycerol lipase Lip5 - Candida rugosa-	An11g00100	SP	yes	87	59	0
115		strong similarity to precursor of tannase - Aspergillus oryzae-	An11g01220	SP	ambiguous	4	1	0
116		s. sim. to triacylglycerol lipase Lip5 - Candida rugosa-	An14g00860	SP	yes	6	52	0
117		s. sim. to lysophospholipase - Aspergillus foetidus-	An16g01880	SP	yes	26	6	4
118		s. sim. to phospholipase B from patent US6146869-A - Aspergillus oryzae-	An16g03700	SP	yes	1	3	6
119		s. sim. to triacylglycerol lipase lipI - Geotrichum candidum-	An16g08870	SP	yes	0	1	0
120		s. sim. to lysophospholipase from patent WO0127251-A - Aspergillus oryzae-	An18g01090	SP	ambiguous	0	1	0
121		s. sim. to hypothetical protein BAC47708.1 - Bradyrhizobium japonicum-	An18g02752	SP	yes	13	14	0
total counts per group						166	176	78

122	ACID	s. sim. to acid phosphatase pho - Pichia pastoris-	An03g05930	SP	yes	0	0	2
123	PHOSPHATASES	s. sim. to phosphate-repressible acid phosphatase precursor phoA - Penicillium chrysogenum-	An08g09850	SP	yes	2	5	3
124		acid phosphatase aph 3-phytase phyB - Aspergillus niger	An08g11030	SP	yes	1	0	2
125		s. sim. to phytase phyA3 - Aspergillus fumigatus-	An12g01910	SP	yes	75	51	7
126		acid phosphatase aphA - Aspergillus niger-	An13g01750	SP	yes	0	0	11
127		s. sim. to acid phosphatase AFPhoA - Aspergillus ficuum-	An14g01550	SP	yes	5	3	0
128		s. sim. to extracellular acid phosphatase PhoA - Penicillium chrysogenum-	An18g04140	SP	yes	1	0	2
total counts per group						84	59	27

129	CELL WALL	s. sim. to filamentous growth protein Dfg5 - Saccharomyces cerevisiae-	An01g06500	SP	yes	0	1	0		Glycoside hydrolase family 76
130	PROTEINS	s. sim. to cell wall protein Crh1 - Saccharomyces cerevisiae-	An01g11010	SP	yes	36	16	36		Glycoside Hydrolase Family 16	yes
131		cerato-platanin	An02g01550	SP	yes	0	0	14
132		s. sim. to hypothetical ECM33 homolog SPCC1223.12c - Schizosaccharomyces pombe-	An04g01230	SP	yes	44	40	51			yes
133		sim. to hypothetical protein MLD14.3 - Arabidopsis thaliana-	An04g07160	SP	likely SP	3	4	6
134		w. sim. to hypothetical cell wall protein binB - Aspergillus nidulans-	An06g00160	SP	yes	0	0	3
135		s. sim. to filamentous growth protein Dfg5 - Saccharomyces cerevisiae-	An06g00360	SP	ambiguous	5	4	0		Glycoside hydrolase family 76	yes
136		s. sim. to cell wall protein Utr2 - Saccharomyces cerevisiae-	An07g07530	SP	yes	40	25	0		Glycoside Hydrolase Family 16	yes
137		sim. to trehlose metabolism factor Pmu1 - Saccharomyces cerevisiae-	An08g02590	SP	yes	13	16	0			yes
138		s. sim. to precursor of glycophospholipid-anchored surface glycoprotein Gas1 - Saccharomyces cerevisiae-	An08g07350	SP	yes	13	4	3		Glycoside Hydrolase Family 72	yes
139		s. sim. to glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferase gel3 - Aspergillus fumigatus-	An09g00670	SP	yes	31	26	7		Glycoside Hydrolase Family 72 and Carbohydrate-Binding Module Family 43	yes
140		s. sim. to 13-beta-glucanosyltransferase gel1 - Aspergillus fumigatus	An10g00400	SP	yes	50	39	22		Glycoside Hydrolase Family 72	yes
141		s. sim. to cell wall protein Crh1 - Saccharomyces cerevisiae-	An11g01540	SP	yes	5	1	0		Glycoside hydrolase family 16
142		s. sim. to hypothetical cell wall protein binB - Aspergillus nidulans-	An14g01820	SP	yes	7	3	118
143		galactomannoprotein; s. sim. to cell wall antigen MP1 - Penicillium marneffei-	An14g02100	SP	yes	5	0	17			yes
144		sim. to protein CAP22 - Colletotrichum gloeosporioides-	An16g01780	SP	yes	0	0	1			yes
145		Allergen Asp F7 homolog	ATCC 128537	No_SP	yes	26	19	11
146		lipid anchored surface protein (GAS1), not in CBS	ATCC 53033	SP	yes	51	43	47		Glycoside Hydrolase Family 72	yes
total counts per group						329	241	336

147	OXIDASES	sim. to hydroquinone oxidase mcrA - Streptomyces lavendulae-	An01g01430	SP	yes	5	7	0
148		s. sim. to catalase cat1 - Aspergillus fumigatus-	An01g01550	SP	yes	11	6	38
149		catalase R catR - Aspergillus niger-	An01g01820	SP	yes	15	56	43
150		w. sim. to tyrosinase melC2 - Streptomyces lincolnensis-	An01g09220	SP	yes	32	50	1
151		s. sim. to laccase I precursor yA - Aspergillus nidulans-	An01g13660	SP	yes	13	0	0
152		s. sim. to carbohydrate oxidase CHO from patent WO9931990-A1 - Microdochium nivale-	An01g14520	SP	yes	0	1	0
153		sim. to hypothetical oxidoreductase ox1 - Claviceps purpurea [putative sequencing error][putative sequencing error]	An02g00460	SP	yes	2	3	0
154		sim. to 6-Hydroxy-D-nicotine oxidase 6-HDNO - Arthrobacter oxidans-	An02g00740	SP	yes	6	6	0
155		s. sim. to 6-hydroxy-D-nicotine oxidase 6-HDNO - Arthrobacter oxidans-	An03g00460	SP	yes	54	30	0
156		s. sim. to isoamyl alcohol oxidase mreA - Aspergillus oryzae-	An03g06270	SP	yes	65	32	0
157		s. sim. to mcrA - Streptomyces lavendulae-	An04g04440	SP	yes	0	1	0
158		sim. to 6-hydroxy-D-nicotine oxidase 6-HDNO - Arthrobacter oxidans-	An05g02390	SP	yes	6	27	0
159		sim. to chloroperoxidase CPO - Caldariomyces fumago-	An06g00720	SP	yes	53	21	15
160		sim. to 6-hydroxy-D-nicotine oxidase 6-HDNO - Arthrobacter oxidans-	An07g02360	SP	yes	70	100	15
161		s. sim. to lactonohydrolase - Fusarium oxysporum-	An08g00490	SP	yes	0	3	0
162		sim. to 6-hydroxy-D-nicotine oxidase 6-HDNO - Arthrobacter oxidans-	An09g01920	SP	yes	0	10	0
163		sulphydryl oxidase Sox from patent EP565172-A1 - Aspergillus niger-	An09g05940	SP	yes	16	29	42
164		s. sim. to isoamyl alcohol oxidase mreA - Aspergillus oryzae-	An11g03740	SP	yes	43	39	0
165		s. sim. to polyamine oxidase PAO - Zea mays-	An13g02480	SP	yes	4	4	0
166		putative oxidioreductase, not in CBS	ATCC 208521	SP	NA	0	4	13
total counts per group						395	429	167

167	HYPOTHETICAL	s. sim. to cytochrome P450 monooxygenase avnA - Aspergillus parasiticus-	An01g00680	SP	ambiguous	0	0	1
168	SP PROTEINS	s. sim. to IgE-binding protein - Aspergillus fumigatus-	An01g06280	SP	yes	0	0	1
169		s. sim. to ribonuclease T2 precursor rntB - Aspergillus oryzae-	An01g10580	SP	yes	11	5	7
170		s. sim. to hypothetical protein CAC28784.2 - Neurospora crassa-	An01g12240	SP	yes	6	3	0
171		sim. to ciscis-muconate lactonizing enzyme I TcMLE - Trichosporon cutaneum	An01g14730	SP	yes	168	79	30
172		s. sim. to asparaginase II Asp3 - Saccharomyces cerevisiae-	An01g14960	SP	yes	34	15	0
173		sim. to enantiomer-selective amidase amdA - Rhodococcus sp.-	An02g00190	SP	yes	24	22	0
174		hypothetical protein-	An02g04060	SP	yes	0	0	2
175		s. sim. to mutarotase enzyme from patent JP07039380-A - Sus scrofa-	An02g09090	SP	likely SP	1	8	24
176		s. sim. to EST an_0208 - Aspergillus niger-	An02g11180	SP	ambiguous	0	0	4
177		hypothetical protein-	An02g11620	SP	yes	0	1	1
178		sim. to hypothetical protein ID880 - Bradyrhizobium japonicum-	An02g13300	SP	yes	0	0	3
179		hypothetical protein-	An02g13650	SP	yes	0	0	6
180		s. sim. to glutaminase A gtaA - Aspergillus oryzae-	An02g13750	SP	yes	8	10	3
181		s. sim. to hypothetical allergic bronchopulmonary aspergillosis allergen rAsp f 4 from patent WO9828624-A1 - Aspergillus fumigatus-	An03g00770	SP	yes	9	6	0
182		hypothetical protein-	An03g01060	SP	yes	0	0	3
183		sim. to almond N-glycosidase from patent EP676473-A2 - Prunus dulcis-	An03g03300	SP	likely SP	2	2	0